منابع مشابه
Spontaneous transfection of mammalian cells with plasmid DNA.
A simple method for spontaneous transfection into mammalian cells (both adherent and suspension in culture) with plasmid DNA is described. This method does not require any specific DNA carrier or technical device and can be applied for obtaining both transient and stably transfected cells. The efficiency of spontaneous transfection is slightly lower in comparison with that of the conventional c...
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The terplex system for delivering DNA to targeted cells comprises three components-stearyl polylysine (PLL), low-density lipoprotein (LDL), and DNA. This article describes a method for optimizing the relative amounts of these components and determining the transfection parameters for a given cell line.
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Near-infrared (NIR) light-responsive liposomes are attractive carriers for targeted and controlled drug delivery to the superficial organ or tissue (e.g., skin). This work describes the development of NIR-responsive liposomes by incorporating gold nanostars within liposomes composed of Phospholipon 90 g and cholesterol. Following cellular delivery, photothermal effect around the gold nanostar u...
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Ultrashort pulsed laser ablation in liquids represents a powerful tool for the generation of pure gold nanoparticles (AuNPs) avoiding chemical precursors and thereby making them especially interesting for biomedical applications. However, because of their electron accepting properties, laser-generated AuNPs might affect biochemical properties of biomolecules, which often adsorb onto the nanopar...
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Efficient non-viral plasmid DNA transfection of most stem cells, progenitor cells and primary cell lines currently presents an obstacle for many applications within gene therapy research. From a standpoint of efficiency and cell viability, magnetic nanoparticle-based DNA transfection is a promising gene vectoring technique because it has demonstrated rapid and improved transfection outcomes whe...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2013
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000113979